Composite

Part:BBa_K4273021:Design

Designed by: Su Junzhe   Group: iGEM22_LINKS_China   (2022-09-30)


pTDH3-Np5598-tTDH1-pPGK1-Np5597-tPGK1-pTEF2-NlmysH-tSSA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3423
    Illegal BglII site found at 5516
    Illegal XhoI site found at 2075
    Illegal XhoI site found at 5936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2321
    Illegal BsaI site found at 5922
    Illegal BsaI.rc site found at 3433
    Illegal BsaI.rc site found at 6172
    Illegal SapI.rc site found at 5741
    Illegal SapI.rc site found at 5862


Design Notes

none


Source

cyanobacteria N. punctiforme and Nostoc linkia NIES-25

References